Tai Lung in Kung Fu Panda uses pressure points to paralyze his victims. (The same technique was also used on him by Master Oogway in the Flashback.) Po turns out to be invulnerable to this, as he is insulated by all his fat. Used in a more comical fashion when a misplaced acupuncture needle causes Po to make a funny face... and maybe stop his heart.
In How to Train Your Dragon, our Badass Bookworm hero Hiccup accidentally discovers through playing with his Dragon friend Toothless that you can render any dragon unconscious with a single finger by pressing an acupuncture point on their necks between the aorta and the larynx.
In Teenage Mutant Ninja Turtles (2012), Master Splinter uses this to calm down and discipline an overreacting Raphael. Later in the episode, Raph himself uses the technique to paralyze a powerful, rampaging mutant.